The isolation of ribounclease B, a glycoprotein, from bovine pancreatic juice.
نویسندگان
چکیده
Greene (1) found that the proteins of bovine pancreatic juice and pancreatic zymogen granules contain identical concentrations of a ribonuclease that on chromatography over Amberlite IRC-50 exhibits the behavior of the enzyme previously called (2, 3) ribonuclease B. The present report deals with the isolation of ribonuclease B from bovine pancreatic juice and provides a description of some of its properties.
منابع مشابه
The Isolation of Ribonuclease B, a Glycoprotein, from Bovine Pancreatic Juice*
Greene (1) found that the proteins of bovine pancreatic juice and pancreatic zymogen granules contain identical concentrations of a ribonuclease that on chromatography over Amberlite IRC-50 exhibits the behavior of the enzyme previously called (2, 3) ribonuclease B. The present report deals with the isolation of ribonuclease B from bovine pancreatic juice and provides a description of some of i...
متن کاملOn the Structure of Bovine Pancreatic Ribonuclease B. Isolation of a Glycopeptide.
Ribonuclease B is a constituent of the zymogen granules of bovine pancreatic acinar cells and is secreted in the pancreatic juice of cattle (2). Its isolation from bovine pancreatic juice has been described (3). The enzyme is a glycoprotein that possesses an amino acid composition indistinguishable from that of ribonuclease A and contains carbohydrate to the extent of 6 residues of mannose and ...
متن کاملOn the Structure of Bovine Pancreatic Ribonuclease B
Ribonuclease B is a constituent of the zymogen granules of bovine pancreatic acinar cells and is secreted in the pancreatic juice of cattle (2). Its isolation from bovine pancreatic juice has been described (3). The enzyme is a glycoprotein that possesses an amino acid composition indistinguishable from that of ribonuclease A and contains carbohydrate to the extent of 6 residues of mannose and ...
متن کاملThe proteins of bovine pancreatic juice.
In the years since the classical work of Northrop and Kunitz on the isolation and characterization of the pancreatic proteins, trypsinogen and chymotrypsinogen-cy (I), an impressive body of knowledge has accumulated about these and other pancreatic proteins. The proteins which have been isolated in crystalline form from the pancreas gland now include trypsinogen, trypsin, chymotrypsinogens a! a...
متن کاملThe protein composition of human pancreatic juice.
1. Human pancreatic juice contains amylase, lipase, ribonuclease, deoxyribonuclease, proelastase, procarboxypeptidase A, procarboxypeptidase B, chymotrypsinogen, and trypsinogen, as well as a tqqsin inhibitor. The level of the inhibitor is such that 1 ml of pancreatic juice containing 4 mg of protein will inhibit about 0.08 mg of bovine trypsin. The inhibitor is a small, basic protein, soluble ...
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ورودعنوان ژورنال:
- The Journal of biological chemistry
دوره 238 شماره
صفحات -
تاریخ انتشار 1963